Rabbit TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

Rabbit TNF-α(Tumor Necrosis Factor Al) ELISA Kit Basic Information

Feiyue's Rabbit TNF-α(Tumor Necrosis Factor Al) ELISA Kit is an ELISA reagent for detection of TNF-α(Tumor Necrosis Factor Al) in Rabbit serum, plasma or with sensitivity, specificity and consistency.TNF-α(Tumor Necrosis Factor Al) IntroductionTumor necrosis factor (TNF, cachexin, or cachectin; often called tumor necrosis factor al or TNF-α) is an adipokine and a cytokine. TNF is a member of the TNF superfamily, which consists of various transmembrane proteins with a homologous TNF domain.TNF was thought to be produced primarily by macroges, but it is produced also by a broad variety of types including lymid , mast , endothelial , cardiac myocytes, adipose tissue, fibroblasts, and neurons.[unreliable medical source? Large amounts of TNF are released in response to lipopolysaccharide, other bacterial products, and interleukin-1 (IL-1). In the skin, mast appear to be the predominant source of pre-formed TNF, which can be released upon inflammatory stimulus. In the brain TNF can protect against excito strengthens synapses. TNF in neurons promotes their survival, whereas TNF in macroges and microglia results in neuronduce apoptosis.TNF-α and IL-6 concentrations are elevated in obesity. Monoclonal antibody against TNF-α is associated with increases rather than decreases in obesity, indicating that inflammation is the result, rather than the cause, of obesity. TNF and IL-6 are the most prominent cytokines predicting COVID-19 severity and death.Rabbit TNF-α(Tumor Necrosis Factor Al) ELISA Kit Test methodThis kit uses sandwich ELISA to detect the concentration of TNF-α(Tumor Necrosis Factor Al) The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNF-α(Tumor Necrosis Factor Al). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TNF-α(Tumor Necrosis Factor Al). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain of TNF-α, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulric acid solution and the color change is measured spectrotometrically at a wavelength of 450nm ± 10nm. The concentration of TNF-α(Tumor Necrosis Factor Al) in the samples is then determined by comparing the OD of the samples to the standard curve.