Quick Details
- Processing Time:3-7days
- Port:Shanghai/shenzhen
- Supply Ability: 300 Bags Per Day
- Brand Name:-
Synonyms: Interleukin-31; IL-31;
IL31
Product name
Human IL-31(Interleukin-31) ELISA Kit
Catalog NO.
FY-EH4753
Reactivity
Human
Assay range
15.6-1,000pg/mL
Sensitivity
< 5.5
pg/mL
Alias
Interleukin-31; IL-31; IL31
Detection Method
Sandwich ELISA,
Double Antibody
Size
48T, 96T
Uniprot ID
Q6EBC2
Storage
2-8 ℃ for 6
months
CV %()
Intra-Assay:
CV<8%
Inter-Assay:
CV<10%
Note
For Research Use
Only
IL-31(Interleukin-31) Basic information
Interleukin-31 (IL-31) is a protein that in humans is encoded by the IL31 gene that resides on chromosome 12. IL-31 is an inflammatory cytokine that helps trigger
cell-mediated immunity against pathogens. It has also been identified as a major player in a number of chronic inflammatory diseases, including atopic
dermatitis. IL-31 is produced by a variety of cells, namely type 2 helper (TH2) T-cells. IL-31 sends signals through a receptor complex made of IL-31RA and oncostatin M receptor β (OSMRβ)
expressed in immune and epithelial cells. These signals activate three pathways: ERK1/2 MAP kinase, PI3K/AKT, and JAK1/2 signaling pathways.IL-31 is believed to play a role
in chronic inflammation diseases. One of these diseases is atopic dermatitis, or eczema. When biopsy samples of patients with atopic dermatitis were compared to samples from patients without
atopic dermatitis, levels of IL-31 were elevated in patients with atopic dermatitis. IL-31 plays a role in this disease by inducing chemokine genes CCL1, CCL17, and CCL22. The chemokines
transcribed from these genes recruit T-cells to the irritated skin where they secrete more IL-31. This cycle is the current understanding of IL-31's role in atopic dermatitis. Along with atopic
dermatitis, IL-31 is believed to play a role in inflammatory bowel disease and airway hypersensitivity.
Pruritic
forms of inflammatory skin diseases, or itchy skin diseases, have been found to have elevated levels of IL-31 mRNA in patient biopsies. Analysis of the tissue distribution of the IL-31 receptor
complex found that IL-31RA is abundant in dorsal root ganglia of different human tissues. Dorsal root ganglia is where the cell bodies of primary sensory neurons reside. Dorsal root ganglia are
also believed to be where the "itch" sensation originates. These findings support the elevated levels of IL-31 in skin biopsies of pruritic skin diseases。
Human IL-31(Interleukin-31) ELISA
Kit test method
Feiyue’s Human IL-31(Interleukin-31) Elisa kit is an ELISA reagent for detection of IL-31(Interleukin-31)
in Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
This kit uses sandwich ELISA to detect the concentRation of Human IL-31(Interleukin-31)
-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex
is formed by additional anti-Human IL-31(Interleukin-31)antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate
reader. The concentRation of Human IL-31(Interleukin-31)in the sample can be calculated by comparing the absorbance of the sample with the standard curve.
Reference:
1, GRCh38: Ensembl release 89: ENSG00000204671 - Ensembl, May 2017
2, "Human PubMed Reference:". National Center for Biotechnology Information,
U.S. National Library of Medicine.
3, "Entrez Gene: interleukin 31".